Program
Time Table
June 6 (Sun)
| Time | Program |
|---|---|
| 2:00 - 10:00 PM | Sign-in |
| 6:00 - 7:00 PM | Dinner |
| 7:30 - 7:40 PM | Opening Remarks - David Mitchell (SUNY Syracuse) |
| 7:45 - 8:45 PM | Keynote Lecture: "Chlamydomonas motility: swimming at the forefront of cilia/flagella
studies" |
| Ritsu Kamiya (The University of Tokyo) | |
| 9:00 - 10:00 PM | Welcome Reception at Balfour-Hood Campus Center |
June 7 (Mon)
| Time | Program | |
|---|---|---|
| Session I. Adaptation and stress responses. | ||
| Chairperson : Stephane Lemaire (CNRS/University Paris-Sud) | ||
| 8:30 - 8:40 AM | Introduction | |
| 8:40 - 8:55 AM | Omer Murik (The Hebrew University of Jerusalem) Paradoxically, prior acquisition of antioxidant activity enhances oxidative stress-induced cell death |
|
| 8:55 - 9:10 AM | Beat Fischer (University of California, Berkeley) Regulation of oxidative stress response in Chlamydomonas reinhardtii |
|
| 9:10 - 9:25 AM | Mariette Bedhomme (CNRS/University Paris-Sud) Thiol-based redox regulation in Chlamydomonas: the emerging role of glutathionylation and glutaredoxins |
|
| 9:25 - 9:40 AM | Dimitris Petroutsos (University of Muenster) The calcium sensing receptor (CAS) and calcium are involved in the high-light response of Chlamydomonas reinhardtii |
|
| 9:40 - 9:55 AM | Eugen Urzica (University of California, Los Angeles) Transcriptome analysis of Chlamydomonas in different iron nutritional conditions |
|
| 9:55 - 10:25 AM | Break | |
| 10:25 - 10:40 AM | Stefan Schmollinger (Max Planck Institute of Molecular Plant Physiology) Modelling of the heat stress response in Chlamydomonas |
|
| 10:40 - 10:55 AM | M. Esther Perez-Perez (CSIC/Instituto de Bioquimica Vegetal y Fotosintesis) Autophagy as an adaptative response to stress in Chlamydomonas reinhardtii |
Session II. Circadian rhythms and cell cycle. |
| Chairperson : Jim Umen (Salk Institute) | ||
| 10:55 - 11:00 AM | Introduction | |
| 11:00 - 11:15 AM | Takuya Matsuo (Nagoya University) Analysis of the circadian clock genes ROCs in Chlamydomonas reinhardtii |
|
| 11:15 - 11:30 AM | Maria Mittag (Friedrich-Schiller University) Multiple roles of an E-box element within the circadian controlled promoter of the c3 gene |
|
| 11:30 - 11:45 AM | Bradley Olson (Salk Institute) Regulation of the cell cycle by a stable nuclear-localized retinoblastoma tumor suppressor complex in Chlamydomonas |
|
| 11:45 - 12:00 AM | Su-chiung Fang (Academia Sinica) A potential sulfate transporter functioning downstream of the RB tumor suppressor pathway in Chlamydomonas |
|
| 12:00 -1:00 PM | Lunch | |
| 1:30 - 3:00 PM | Poster Session A | |
| Session llI. Flagellar structure and function. | ||
| Chairperson : Mary Porter (University of Minnesota) | ||
| 3:00 - 3:10 PM | Introduction | |
| 3:10 - 3:25 PM | Pinfen Yang (Marquette University) RSP3 contains two distinct sites for anchoring RIIa and DPY-30 clan members in the radial spoke complex |
|
| 3:25 - 3:40 PM | Candice Elam (Emory University School of Medicine) The Chlamydomonas paralyzed flagellar mutant pf4 is defective in the PP2A B-subunit and reveals that PP2A is required for normal motility and phototaxis |
|
| 3:40 - 3:55 PM | Mary Porter (University of Minnesota) New insights into the nexin-dynein regulatory complex |
|
| 3:55 - 4:05 PM | Maureen Wirschell (Emory University School of Medicine) PF3 encodes a DRC subunit required for assembly of the DRC-nexin link |
|
| 4:05 - 4:20 PM | Break | |
| 4:20 - 4:30 PM | Anne Gaillard (Sam Houston State University) Select acetophenones alter flagellar motility in Chlamydomonas |
|
| 4:30 - 4:45 PM | Jens Boesger (Friedrich Schiller University Jena) Comparative phosphoproteome analysis suggests targets of casein kinase 1 in Chlamydomonas renhardtii flagella |
|
| 4:45 - 5:00 PM | Hitoshi Sakakibara (Kobe Adv Inst Inf Comm Tech) Nano-dissection of inner-arm dynein-c by high-speed AFM |
|
| 5:00 - 5:15 PM | David Mitchell (SUNY Upstate Medical University) Cytoplasmic factors needed for axonemal dynein assembly |
|
| 5:15 - 5:30 PM | Antonio Castillo-Flores (University of Massachusetts Medical School) The flagellar membrane of Chlamydomonas is a specialized domain of the plasma membrane enriched in raft lipids |
|
| 5:30 - 6:30 PM | Dinner | |
| Workshop 1. Omics Approaches and Biotechnology. | ||
| Chairperson : Michael Hippler (University of Muenster) | ||
| 7:00 - 7:06 PM | Introduction | |
| 7:06 - 7:24 PM | Mia Terashima (University of Muenster) Characterization of a novel candidate gene involved in the anaerobic response of Chlamydomonas reinhardtii as revealed by comparative proteomics |
|
| 7:24 - 7:42 PM | Michael Schroda (Max Planck Inst Mol Plant Phys) The GoFORSYS Systems Biology project - quantitative shotgun proteomics as a tool for monitoring proteome dynamics on Chlamydomonas |
|
| 7:42 - 7:54 PM | Robert L. Morris (Wheaton College) Developmental regulation of the ciliary proteome in sea urchin |
|
| 7:54 - 8:06 PM | Neil D. Clarke (Genome Institute of Singapore) Systems biology of lipid metabolism in Chlamydomonas |
|
| 8:06 - 8:24 PM | Hideya Fukuzawa (Kyoto University) Visualization of digital expression profiles on the nuclear genome of the mating-type minus strain by Kyoto Chlamydomonas Genome Database (KCGD): Expression from the mating-type locus and CO2-limiting stress responses |
|
| 8:24 - 8:36 PM | Simon E. Alfred (University of Toronto) High-throughput identification of biologically active small molecules on Chlamydomonas |
|
| 8:36 - 8:48 PM | Michael Hippler (University of Muenster) Improving gene models of Chlamydomonas reinhardtii by mass spectrometric data from Proteomics experiments |
|
| 8:48 - 9:00 PM | Olivier Vallon (IBPC CNRS) Discussion on gene annotation of the JGI database |
|
June 8 (Tue)
| Time | Program | |
|---|---|---|
| Session lV. IFT and flagellar assembly. | ||
| Chair : Pete Lefebvre (University of Minnesota) | ||
| 8:30 – 8:35 AM | Introduction | |
| 8:36 - 8:50 AM | Carolyn Silflow (University of Minnesota) The Chlamydomonas VFL3 gene is essential for normal segregation and positioning of basal bodies |
|
| 8:50 – 9:04 AM | Alison Albee (Washington University) Elucidating the function of Lnc1, a phopholipase B |
|
| 9:04 – 9:18 AM | Alan Kwan (Washington University) RNA-seq of Chlamydmonas deflagellation reveals early genetic program of ciliogenesis |
|
| 9:18 – 9:32 AM | Lynne Quarmby (Simon Fraser University) Is there a relationship between deflagellation and flagellar resorption? |
|
| 9:32 – 9:46 AM | Branch Craige (University of Massachusetts Medical School) CEP290 is a dynamic transition zone protein required for tethering the membrane to the transition zone microtubules, normal intraflagellar transport (IFT), and flagellar assembly |
|
| 9:46 – 10:00 AM | Lai-Wa Tam (University of Minnesota) iTRAQ analysis of lf2 mutant flagella identifies new candidate proteins involved in IFT and flagellar length control |
|
| 10:00 – 10:30 AM | Break | |
| 10:30 – 10:44 AM | Junmin Pan (Tsinghua University) Control of flagellar assembly and disassembly by a microtubule depolymerase CrKinesin13 |
|
| 10:44 - 10:58 AM | William Ludington (University of California San Francisco) Revisiting the long-zero response: measurement of the flagellar growth and shortening rates in live, motile cells |
|
| 10:58 - 11:12 AM | Douglas Cole (University of Idaho) IFT121 and IFT 122 bind to IFT43 and are required for flagellar assembly |
|
| 11:12 - 11:26 AM | Nedra Wilson (Oklahoma State University) Characterization of the flagellar form of LF4p, a MAP kinase involved in the regulation of flagellar length |
|
| 11:26 - 11:40 AM | Karl-Ferdinand Lechtreck (University of Massachusetts Medical School) Defects in Bardet-Biedl syndrome (BBS) genes impair Chlamydomonas phototaxis |
|
| 11:40 – 11:54 AM | Kaiyao Huang (Yale University) The ubiquitin conjugation system is involved in the disassembly of cilia/flagella |
12:00 -1:00 PM | Lunch |
| 1:30 - 3:00 PM | Poster Session A | |
| Session V. Evolution, Development and Life Cycle. | ||
| Chairs: Aurora Nedelcu (Univ New Brunswick) Ursula Goodenough (Washington Univ) | ||
| 3:00 - 3:10 PM | Introduction | |
| 3:10 - 3:25 PM | Katherine E. Helliwell (University of Cambridge) Methionine synthase isoform is a key determinant in the evolution of vitamin B12 auxotrophy in algae |
|
| 3:25 - 3:40 PM | Jae-Hyeok Lee (Washington University) Identification and characterization of hydroxyproline-rich glycoprotein networks for building cell walls in the green lineage |
|
| 3:40 - 3:55 PM | Yanjie Liu (Univ Texas Southwestern Medical Center) Membrane fusion triggers rapid degradation of two gamete-specific, fusion-essential proteins in a membrane block to polygamy in Chlamydomonas |
|
| 3:55 - 4:10 PM | James Umen (The Salk Institute) Evolution of an expanded sex determining region in Volvox |
|
| 4:10 - 4:25 PM | Aurora Nedelcu (University of New Brunswick) General acclimation responses co-opted for somatic cell differentiation in Volvox carteri |
|
| 4:25 - 4:40 PM | Break | |
| Session VI. Gene Regulation. | ||
| Chairperson : Sabeeha Merchant (University of California, Los Angeles) | ||
| 4:40 - 4:50 PM | Introduction | |
| 4:50 - 5:05 PM | Daniela Strenkert (Max Planck Institute for Molecular Plant Physiology) Transcription factor dependent rearrangement of chromatin structure as a regulatory mechanism controlling gene expression at different environmental conditions in Chlamydomonas reinhardtii |
|
| 5:05 - 5:20 PM | Tomohito Yamasaki (University of Nebraska) RNA interference mediated by translation repression and the role of raptor, the regulatory associated protein of TOR (target of rapamycin) |
|
| 5:20 - 5:35 PM | Andrew Bassett (University of Cambridge) A screen for sRNA-directed chromatin silencing in Chlamydomonas |
|
| 5:35 - 6:30 PM | Dinner | |
| 7:00 - 8:00 PM | Invited Lecture: "Choanoflagellates and the origin of animals" |
|
| Nicole King (University of California, Berkeley) | ||
| 8:00 - 9:00 PM | Invited Lecture:"From comparative genomics to the bench: insights on the green alga Micromonas" |
|
| Alexandra Worden (Monterey Bay Aquarium Research Institute) | ||
June 9 (Wed)
| Time | Program | |
|---|---|---|
| Session VII. Photosynthesis. | ||
| Chairperson : Bob Spreitzer (University of Nebraska) | ||
| 8:30 - 8:40 AM | Introduction | |
| 8:40 - 8:55 AM | Martin H. Spalding (Iowa State University) Role of LCIB in Chlamydomonas limiting CO2 acclimation: Localization and interactions | |
| 8:55 - 9:10 AM | Jun Minagawa (Hokkaido University) Imaging state-transitions visualized energy-dissipative light-harvesting complexes in photosynthesis | |
| 9:10 - 9:25 AM | Elisabeth Ostendorf (WWU Muenster) Localization of the qE required light-harvesting complex protein LhcSR3 | |
| 9:25 - 9:40 AM | Amina Antonacci (Institute of Crystallography, CNR, Rome) Single amino acidic substitutions in the Chlamydomonas reinhardtii D1 protein affect photosynthesis efficiency, carotenoids and plastoquinone biosynthesis in physiological and stressful conditions | |
| 9:40 - 10:05 AM | Break | |
| Session VIII. Light Perception and Photoresponses. | ||
| Chairperson : Georg Kreimer (Friedrich-Alexander-University Erlangen) | ||
| 10:05 - 10:10 AM | Introduction | |
| 10:10 - 10:25 AM | Joseph Boyd (University of Arizona) Analysis and characterization of novel Chlamydomonas reinhardtii eyespot mutants |
|
| 10:25 - 10:40 AM | Thomas Schulze (Friedrich-Schiller University) Functional characterization of the eyespot protein SOUL3 in Chlamydomonas reinhardtii |
|
| 10:40 - 10:55 AM | Peter Hegemann (Humboldt University, Berlin) Channelrhodopsin, insight into the light switch |
|
| 10:55 - 11:10 AM | Andre Greiner (Humboldt University, Berlin) Function and processing of Chlamydomonas phototropin |
|
| 11:10 - 11:20 AM | Jason Peterson (Avera Research Institute, Sioux Falls) The essential role of 7,8-didemethyl-8-hydroxy-5-deazariboflavin for optimal photoreactivation in Chlamydomonas reinhardtii |
|
| 11:30 -4:30 PM | Lunch and Free Afternoon; Excursion | |
| 4:30 - 6:00 PM | Poster Session B | |
| 6:00 - 7:30 PM | Banquet | |
| Workshop 2. Genetics and tools. | Chairperson : Susan Dutcher (Washington University) | |
| 8:00 - 8:05 PM | Introduction | |
| 8:05 - 8:15 PM | Elizabeth Harris, Pete Lefebvre and Matt Laudon Chlamydomonas Genetics Center Update |
|
| 8:15 - 8:30 PM | Michael Moulin (University of Cambridge) Riboswitch as molecular tool for controling artificially gene expression |
|
| 8:30 - 8:45 PM | Stefan Schmollinger (Max Planck Institute of Mol. Plant Physiology) Development of an inducible artificial microRNA system for Chlamydomonas reinhardtii as a tool for investigation of essential gene function |
|
| 8:45 - 9:00 PM | Olivier Vallon (IBPC CNRS) New tools for insertional mutagenesis |
|
| 9:00 - 9:15 PM | Huawen Lin (Washington University) A mutant in the NimA-like kinase, cnk10, has flagellar and cell cycle defects |
|
| 9:15 - 9:30 PM | Irina Sizova (St. Petersburg Nucl. Phys. Inst.) Construction of zinc finger nucleases for targeting of the Chlamydomonas ChR1 gene |
|
| 9:30 - 9:45 PM | Krishna Niyogi (University of California, Berkeley) Reverse genetics of Chlamydomonas using TILLING |
|
June 10 (Thu)
| Time | Program | |
|---|---|---|
| Session IX. Biofuels and Metabolism. | ||
| Chairperson : Beth Rasala (Scripps Institute) | ||
| 8:30 - 8:40 AM | Introduction | |
| 8:40 - 8:55 AM | Zi Wang (Washington University) The influence of acetate, CO2, and light on nitrogen-stress-induced formation of lipid bodies and 13C flux in C. reinhardtii |
|
| 8:55 - 9:10 AM | Rachel Miller (Michigan State University) Triacylglycerol biosynthesis in Chlamydomonas reinhardtii |
|
| 9:10 - 9:25 AM | Ursula Goodenough (Washington University) The cw15 sta6 mutant strain of C. reinhardtii produces lipid bodies in both the cytoplasm and the chloroplast in response to nitrogen starvation in 20 mM acetate |
|
| 9:25 - 9:40 AM | Anja Hemschemeier (Ruhr University Bochum) Hydrogen production in Chlamydomonas reinhardtii: new insights into the biochemistry of a photosynthetic FeFe-hydrogenase |
|
| 9:40 - 9:55 AM | Jorg Toepel (Bielefeld University) System biology approach to increase hydrogen production in C. reinhardtii |
|
| 9:55 - 10:25 AM | Break | |
| 10:25 - 10:40 AM | Sonia Cruz (University of Cambridge) Using Chlamydomonas reinhardtii to generate electrical current: insight into the cell wall and cell membranes proteomics |
|
| 10:40 - 10:55 AM | Jason Papin (University of Virginia) Genome-wide network analysis of metabolism in Chlamydomonas reinhardtii |
|
| 10:55 - 11:10 AM | Lila Ghamsari (Dana-Farber Cancer Institute) Identifying the metabolic potential of Chlamydomonas reinhardtii by large-scale annotation of its encoded open reading frames |
|
| 11:10 - 11:25 AM | Claude Aflalo (Ben Gurion University) Insight in microalgal carbon flux under growth and stress conditions by biomass analysis |
|
| 11:25 - 11:40 AM | Elena Kazamia (University of Cambridge) Dynamics of algal-bacterial interactions: prospects for large scale outdoor cultivation of algae for biofuel production |
|
| 12:00-1:00 PM | Lunch | |
| 1:30 - 3:00 PM | Poster Session B | |
| Session X. Chloroplast Function and Biogenesis. | ||
| Chairperson : David Stern (Cornell University) | ||
| 3:00 - 3:10 PM | Introduction | |
| 3:10 - 3:25 PM | Yuichiro Takahashi (Okayama University) Determination of stoichiometry of the five multiprotein complexes in the thylakoid membranes |
|
| 3:25 - 3:45 PM | Andre Nordhues (Max Planck Institute of Molecular Plant Physiology) Function of VIPP1 (vesicle-inducing protein in plastids) |
|
| 3:45 - 4:05 PM | Lutz Wobbe (University of Bielefeld) Cytosolic translation control of LHCII mRNAs |
|
| 4:05 - 4:25 PM | Break | |
| 4:30 - 4:50 PM | Yves Choquet (Institute of Biology Physicochemistry, CNRS-UPMC) Turnover of MCA1, the stabilization factor of petA mRNA, is coupled to the rate of translation of petA through its interaction with newly synthesised cytochrome f |
|
| 4:45 - 5:00 PM | Phoi Tran (UC Berkeley) Intragenic enhancers and suppressors of phytoene desaturase mutations |
|
| 5:00 - 5:15 PM | Jessica Jacobs (Ruhr-University Bochum) Components of a putative spliceosome involved in chloroplast RNA trans-splicing |
|
| 5:15 - 5:30 PM | Silvia Ramundo (University of Geneva) Conditional vitamin-mediated repression of chloroplast gene expression |
|
| 5:30 - 6:30 PM | Dinner | |
| 7:15 - 7:30 PM | Poster Awards, Announcements | |
| 7:30 - 8:30 PM | Invited Lecture: "Intraflagellar Transport: it's not just for cilia any more" |
|
| Joel Rosenbaum (Yale University) | 8:30 - 8:45 PM | Closing Remarks - Stephen Miller (Univ of Maryland, Baltimore County) |
June 11 (Fri)
Light Breakfast and Return home